HPLC/UPLC diagnostic

Please try to fill in this diagnostic. It is meant to assess confidence and how we can help. We are happy to provide a review of topics you might understand; however, we don’t want you sitting through materials you already understand.

I know what isocratic means. *YesNo

I know what gradients are in LC. *YesNo

I am confident using spreadsheets for basic computations (=, +, -, *, /). *YesNo

I am confident extracting peak areas and using them. *YesNo

I can prepare a stock standard solution. *YesNo

I can prepare a set of standards from a stock solution to calibrate an LC. *YesNo

I could specify a quantitative HPLC method for sugars. *YesNo

I could specify a quantitative LC method for organic acids. *YesNo

I would be confident measuring caffeine by LC. *YesNo

I can specify a quantitative LC method for nitrate. *YesNo

I know how to prepare samples for LC. *YesNo

I understand HPLC vs. UPLC. *YesNo

I can name at least 6 detectors for LC from memory. *YesNo

I understand when I might use each of the six detectors and can give an example. *YesNo

I know how to load HPLC samples. *YesNo

I feel confident using an HPLC. *YesNo

I could change an HPLC column and do routine checks. *YesNo

I could set up and troubleshoot an HPLC. *YesNo

I could estimate costs of running a hundred samples by HPLC. *YesNo

I understood how SPE cartridges might fit into an HPLC method. *YesNo

I can calculate the number of plates and plate height from a chromatogram. *YesNo

I can calculate resolution from a chromatogram. *YesNo

I understand how thermodynamic and kinetics influence LC. *YesNo

I understand the van Deemter equation and how it applies to LC. *YesNo

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