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HPLC/UPLC diagnostic (No Lab)

Please try to fill in this diagnostic. It is meant to assess confidence and how we can help. We are happy to provide a review of topics you might understand; however, we don’t want you sitting through materials you already understand.

I know what isocratic means. *
I know what gradients are in LC and how to use them. *
I am confident using spreadsheets for basic computations (=, +, -, *, /). *
I am confident extracting peak areas and using them. *
I can prepare a stock standard solution. *
I can prepare a set of standards from a stock solution to calibrate an LC. *
I could specify a quantitative HPLC method for sugars. *
I could specify a quantitative LC method for organic acids. *
I would be confident measuring caffeine by LC. *
I can specify a quantitative LC method for nitrate. *
I know how to prepare samples for LC. *
I understand HPLC vs. UPLC. *
I can name at least 6 detectors for LC from memory. *
I understand when I might use each of the six detectors and can give an example. *
I could estimate costs of running a hundred samples by HPLC. *
I understood how SPE cartridges might fit into an HPLC method. *
I can calculate the number of plates and plate height from a chromatogram. *
I can calculate resolution from a chromatogram. *
I understand how thermodynamic and kinetics influence LC. *
I understand the van Deemter equation and how it applies to LC. *
Interested in being on our mailing list? *
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